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Advanced techniques for gene heterogeneity research: Single-cell sequencing and on-chip gene analysis systems

Abstract Gene heterogeneity leads to the differences in cellular behaviors in a wide range, such as tumor drug-resistant mutation, epithelial-mesenchymal transition, and migration, posing significant challenges to the development of biomedicine. Traditional gene analysis methods, such as polymerase chain reaction, employ a mass of cells as the gene source, resulting in that the gene properties [...]

Time-efficient fabrication method for 3D-printed microfluidic devices

Abstract Recent developments in 3D-printing technology have provided a time-efficient and inexpensive alternative to the fabrication of microfluidic devices. At present, 3D-printed microfluidic systems face the challenges of post-processing, non-transparency, and being time consuming, limiting their practical application. In this study, a time-efficient and inexpensive fabrication method was developed for 3D-printed microfluidic devices. The material [...]

Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture

1 Introduction To understand interactions between living matter and artificial materials, tissue engineering research groups focus on the development of 3D scaffolds with increasingly controllable properties, reaching from biochemical interactions at molecular scale to nanoscale hydrogel networks, micrometer-scale pores, and macroscopic architectures.[1] Soft hydrogel scaffolds are conventionally formed by crosslinking a precursor solution, resulting in soft, [...]

Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry

Abstract Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific functional networks underlying biological processes. Here, we present a streamlined workflow combining microfluidic chips for all-in-one proteomic sample preparation and data-independent acquisition (DIA) mass spectrometry (MS) for proteomic analysis down to the single-cell level. The proteomics chips enable multiplexed and automated cell isolation/counting/imaging and sample [...]